Development of an optimal method for the dual perfusion of the isolated rat liver.

@article{ahin1998DevelopmentOA,
  title={Development of an optimal method for the dual perfusion of the isolated rat liver.},
  author={Selma Şahin and Malcolm Rowland},
  journal={Journal of pharmacological and toxicological methods},
  year={1998},
  volume={39 1},
  pages={35-43}
}
The liver receives two blood supplies, the portal vein (PV) and hepatic artery (HA). The isolated perfused rat liver preparation (IPRL) is widely used to examine physiological factors affecting the hepatic disposition of compounds, but usually it is perfused via the PV only. In the development of a more physiological dual perfused system, we examined three surgical procedures for HA perfusion--cannulation through the gastroduodenal artery, the aorta, and the celiac artery--using 14C-urea as the… CONTINUE READING