Development of an integrative vector for the expression of antisense RNA in Clostridium difficile.

@article{Roberts2003DevelopmentOA,
  title={Development of an integrative vector for the expression of antisense RNA in Clostridium difficile.},
  author={Adam Paul Roberts and Claire Hennequin and Mike Elmore and A. M. Collignon and Tuomo K. Karjalainen and Nigel P. Minton and Peter Mullany},
  journal={Journal of microbiological methods},
  year={2003},
  volume={55 3},
  pages={617-24}
}
A method was developed to use the conjugative transposon Tn916 as a vector for introducing recombinant DNA into Clostridium difficile. This was used to introduce antisense RNA for the adhesin encoding gene cwp66 into C. difficile 79-685. RT-PCR demonstrated that cwp66 specific antisense RNA was produced. However, there was no statistically significant difference in the protein expression or in the adherence of recombinant C. difficile strains. This may be due to the amount of transcripts of the… CONTINUE READING