Development of an integration mutagenesis system in Lactobacillus gasseri.

@article{Selle2014DevelopmentOA,
  title={Development of an integration mutagenesis system in Lactobacillus gasseri.},
  author={Kurt Selle and Yong Jun Goh and Sarah O'Flaherty and Todd R. Klaenhammer},
  journal={Gut microbes},
  year={2014},
  volume={5 3},
  pages={326-32}
}
Lactobacillus gasseri ATCC 33323 is a member of the acidophilus-complex group, microbes of human origin with significant potential for impacting human health based on niche-specific traits. In order to facilitate functional analysis of this important species, a upp-based counterselective chromosomal integration system was established and employed for targeting the lipoteichoic acid (LTA) synthesis gene, ltaS, in L. gasseri ATCC 33323. The ltaS gene encodes a phosphoglycerol transferase… CONTINUE READING