Development of an indirect ELISA method based on the VP3 protein of duck hepatitis A virus type 1 (DHAV-1) for dual detection of DHAV-1 and DHAV-3 antibodies.

Abstract

An indirect enzyme-linked immunosorbent assay (I-ELISA) based on the recombinant VP3 protein of duck hepatitis A virus type 1 (DHAV-1) was developed and evaluated in this study. The optimal antigen, serum and enzyme-labeled antibody dilutions were 1:160 (0.94μg), 1:160 and 1:2000, respectively. The optimal blocking buffer was 1% gelatin. The cutoff value… (More)
DOI: 10.1016/j.jviromet.2015.08.016

Topics

2 Figures and Tables

Cite this paper

@article{Shen2015DevelopmentOA, title={Development of an indirect ELISA method based on the VP3 protein of duck hepatitis A virus type 1 (DHAV-1) for dual detection of DHAV-1 and DHAV-3 antibodies.}, author={Youlin Shen and Anchun Cheng and Mingshu Wang and Shun Chen and Renyong Jia and Dekang Zhu and Mafeng Liu and Kunfeng Sun and Qiao Yang and Xiaoyue Chen}, journal={Journal of virological methods}, year={2015}, volume={225}, pages={30-4} }