Development of an in vitro skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol.

@article{Ashikaga2006DevelopmentOA,
  title={Development of an in vitro skin sensitization test using human cell lines: the human Cell Line Activation Test (h-CLAT). I. Optimization of the h-CLAT protocol.},
  author={Takao Ashikaga and Yukiko Yoshida and Morihiko Hirota and Katsurako Yoneyama and Hiroshi Itagaki and Hitoshi Sakaguchi and Masaaki Miyazawa and Yuichi Ito and Hiroyuki Suzuki and Hidekazu Toyoda},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={2006},
  volume={20 5},
  pages={767-73}
}
The aim of this study is to optimize the experimental conditions for an in vitro skin sensitization test using the human cell lines THP-1 and U-937. As regards pre-culturing time, the expression of CD86 on DNCB-treated THP-1 cells tended to be higher after 48h and 72h pre-culture compared with other time points evaluated. Next, we investigated the effect of chemical treatment time, and found that induction of CD86 expression on THP-1 cells by DNCB reached a plateau after 24h. Augmentation of… CONTINUE READING

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