Development of an in vitro photosensitization assay using human monocyte-derived cells.

@article{Hoya2009DevelopmentOA,
  title={Development of an in vitro photosensitization assay using human monocyte-derived cells.},
  author={Masatoshi Hoya and Morihiko Hirota and Mie Suzuki and Shigenobu Hagino and Hiroshi Itagaki and Setsuya Aiba},
  journal={Toxicology in vitro : an international journal published in association with BIBRA},
  year={2009},
  volume={23 5},
  pages={
          911-8
        }
}
In this study, with the aim of developing a cell-based in vitro photosensitization assay, we examined whether changes of CD86 and CD54 expression on cells of a human monocytic cell line, THP-1, could be used to assess the photosensitizing potential of chemicals. First, we identified suitable conditions of UV-irradiation (irradiation dose; 5.0 J/cm(2), irradiation intensity; 1.7 mW/cm(2)) by investigating the effect of UV-irradiation on CD86 and CD54 expression on untreated or 6-methylcoumarin… CONTINUE READING

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