Development of an immunofluorescence technique for detecting Prorocentrum donghaiense Lu

  title={Development of an immunofluorescence technique for detecting Prorocentrum donghaiense Lu},
  author={Da-zhi Wang and Xu-guang Huang and Leo W L Chan and Hua-sheng Hong},
  journal={Journal of Applied Phycology},
Prorocentrum donghaiense Lu is a key harmful algal bloom (HAB) species which is widespread along the China Sea coast. In this study, P. donghaiense-specific antiserum was developed, and the detection method, based on immunofluorescence (IF), was optimized. The antiserum was raised using 0.5% paraformaldehyde-fixed whole cells (WC) as antigen. The titer and the specificity were examined using whole-cell IF. Results showed that ethanol was an effective decolorization reagent, and 80% ethanol was… CONTINUE READING


Publications referenced by this paper.
Showing 1-10 of 17 references

Development of an immunofluorescence technique for detecting Pfiesteria piscicida

  • SJ Lin, TN Feinstein, H Zhang
  • Harmful Algae
  • 2003
Highly Influential
4 Excerpts

Five red tide species in genus Prorocentrum including the description of Prorocentrum donghaiense Lu sp. nov. from the East China Sea

  • DD Lu, J Goebel
  • Chin J Oceanol Limnol
  • 2001
Highly Influential
4 Excerpts

Immunochemical characterization of morphospecies and strains of Prorocentrum (Dinophyceae)

  • V Lopez-Rodas, E Costas
  • J Exp Mar Bio Ecol
  • 1999
Highly Influential
4 Excerpts

The use of polyclonal antisera and blocking of antibodies in the identification of marine dinoflagellates: species-specific and clone-specific antisera against Gymnodinium and Alexandrium

  • H Mendoza, V Lopez-Rodas, F Gonzalez-Gil, E Costas
  • J Exp Mar Biol Ecol
  • 1995
Highly Influential
8 Excerpts

Discrimination between domoic-acid-producing and nontoxic forms of the diatom Pseudonitzschia pungens using immunofluorescence

  • M Bates, C Léger, BA Keafer, DM Anderson
  • Mar Ecol Prog Ser
  • 1993
Highly Influential
5 Excerpts

The identification of conspecific dinoflagellates Alexandrium tamarense from Japan and Thailand by monoclonal antibodies

  • M Adachi, Y Sako, Y Ishida
  • Nippon Suisan Gakkaishi
  • 1993
Highly Influential
7 Excerpts

Immunofluorescent detection of the brown tide organism Aureococcus anophagefferens

  • DM Anderson, DM Kulis, EM Cosper
  • Cosper EM, Bricelj VM,
  • 1990
Highly Influential
6 Excerpts

Immunofluorescence in phytoplankton research: applications and potential

  • EG Vrieling, DM Anderson
  • J Phycol
  • 1996
Highly Influential
4 Excerpts

Identification of harmful algal species using molecular probes: an emerging technology

  • DM Anderson
  • 1995
Highly Influential
7 Excerpts

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