Development of an immunoassay to measure the humoral immune response of hybrid striped bass Morone chrysops x M. saxatilis.

@article{Shelby2003DevelopmentOA,
  title={Development of an immunoassay to measure the humoral immune response of hybrid striped bass Morone chrysops x M. saxatilis.},
  author={Richard A Shelby and Craig A Shoemaker and Phillip H. Klesius},
  journal={Veterinary immunology and immunopathology},
  year={2003},
  volume={91 3-4},
  pages={217-25}
}
Hybrid striped bass (HSB) were immunized with bovine serum albumin (BSA) and the specific anti-BSA immunoglobulin (Ig) was affinity purified from the resulting serum by means of an agarose gel-BSA column. The native Ig had an apparent molecular size of 893 KDD, by size exclusion chromatography, and when examined by polyacrylamide gel electrophoresis (PAGE) under denaturing conditions, resolved to heavy (H) and light (L) chains of 76 and 27 KDD, respectively. Affinity purified native HSB Ig was… CONTINUE READING