Development of an enzyme-linked immunosorbent assay for quantifying vitellogenin in Pacific salmon and assessment of field exposure to environmental estrogens.

@article{Peck2011DevelopmentOA,
  title={Development of an enzyme-linked immunosorbent assay for quantifying vitellogenin in Pacific salmon and assessment of field exposure to environmental estrogens.},
  author={Karen A Peck and Daniel P Lomax and Ordean Olson and Sean Y Sol and Penny Swanson and Lyndal L. Johnson},
  journal={Environmental toxicology and chemistry},
  year={2011},
  volume={30 2},
  pages={477-86}
}
A competitive enzyme-linked immunosorbent assay was developed to quantitate vitellogenin (VTG) in plasma and serum of coho (Oncorhynchus kisutch) and chinook (O. tshawytscha) salmon. The working range of the assay was 9 to 313 ng/ml (80-20% binding), with 50% binding at 54 ng/ml. The intra-assay and interassay variations at approximately 50% binding were 8.1% (n = 9) and 9.0% (n = 9), respectively. Dilution curves of plasma or serum from coho and chinook females and estrogen-treated males were… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…