Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp.

@article{Yin2014DevelopmentOA,
  title={Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp.},
  author={Jin Yin and Xiao-Zhi Fu and Qiong Wu and Jinchun Chen and Guo-Qiang Chen},
  journal={Applied Microbiology and Biotechnology},
  year={2014},
  volume={98},
  pages={8987-8997}
}
Since halophile Halomonas spp. can grow contamination free in seawater under unsterile and continuous conditions, it holds great promise for industrial biotechnology to produce low-cost chemicals in an economic way. Yet, metabolic engineering methods are urgently needed for Halomonas spp. It is commonly known that chromosomal expression is more stable yet weaker than plasmid one is. To overcome this challenge, a novel chromosomal expression method was developed for halophile Halomonas TD01 and… 
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