Development of an electroporation procedure for gene disruption in Lactobacillus helveticus CNRZ 32

@inproceedings{Bhowmik1993DevelopmentOA,
  title={Development of an electroporation procedure for gene disruption in Lactobacillus helveticus CNRZ 32},
  author={Tarun Bhowmik and James L. Steele},
  year={1993}
}
Summary: An electroporation-mediated transformation method was developed and optimized for Lactobacillus helveticus CNRZ 32. The effects of electroporation buffers, growth conditions, cell-wall-weakening agents and field strength on transformation frequency were examined. Optimal conditions yielded a frequency of 2 × 104 transformants (μg pGK12)−1. Using this procedure, an integration plasmid was introduced into L. helveticus CNRZ 32 to inactivate the chromosomal X-prolyl dipeptidyl… CONTINUE READING

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