Development of an electrochemical Limulus amebocyte lysate assay technique for portable and highly sensitive endotoxin sensor

@article{Inoue2012DevelopmentOA,
  title={Development of an electrochemical Limulus amebocyte lysate assay technique for portable and highly sensitive endotoxin sensor},
  author={Kumi Y Inoue and Satoko Takahashi and Kosuke Ino and Hitoshi Shiku and Tomokazu Matsue},
  journal={Innate Immunity},
  year={2012},
  volume={18},
  pages={343 - 349}
}
Here, we report the development of an electrochemical detection method for endotoxin based on the Limulus amebocyte lysate (LAL) assay. A mixture of LAL reagent and endotoxin sample solution was incubated for 1 h. The endotoxin activated a cascade reaction of zymogens contained in the LAL to generate p-nitroaniline (pNA) which was then electrochemically detected by differential pulse voltammetry (DPV). The generated pNA gave a clear peak at –0.75 V vs. silver/silver chloride (Ag/AgCl), which… Expand
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