Development of an efficient regeneration and Agrobacterium-mediated transformation system in crab apple (Malus micromalus) using cotyledons as explants

@article{Dai2013DevelopmentOA,
  title={Development of an efficient regeneration and Agrobacterium-mediated transformation system in crab apple (Malus micromalus) using cotyledons as explants},
  author={Hongyan Dai and Wenran Li and Wenjuan Mao and Lei Zhang and Guofen Han and Kai Zhao and Yuexue Liu and Zhihong Zhang},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2013},
  volume={50},
  pages={1-8}
}
Apple has become a model species for Rosaceae genetic and genomic research, but it is difficult to obtain transgenic apple plants by Agrobacterium-mediated transformation using in vitro leaves as explants. In this study, we developed an efficient regeneration and Agrobacterium-mediated transformation system for crab apple (Malus micromalus) using cotyledons as explants. The proximal cotyledons of M. micromalus, excised from seedlings that emerged from mature embryos cultured for 10–14 d in… CONTINUE READING