Development of an efficient on-chip digestion system for protein analysis using MALDI-TOF MS.

Abstract

A solid-phase trypsin microreactor was constructed and operated with electrokinetically-driven flow for the digestion of proteins and coupled off-line with MALDI-TOF MS. The bioreactor was fabricated from poly(methyl methacrylate), PMMA, by hot embossing using a mold master prepared by micro-milling. The solid-phase bioreactor consisted of a 4 cm long, 200… (More)
DOI: 10.1039/b916556h

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@article{Lee2009DevelopmentOA, title={Development of an efficient on-chip digestion system for protein analysis using MALDI-TOF MS.}, author={Jeonghoon Lee and Steven Allan Soper and Kermit K Murray}, journal={The Analyst}, year={2009}, volume={134 12}, pages={2426-33} }