Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis

@article{Guan2016DevelopmentOA,
  title={Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis},
  author={Chengran Guan and Wenjing Cui and Jintao Cheng and Li Zhou and Zhongmei Liu and Zhemin Zhou},
  journal={Microbial Cell Factories},
  year={2016},
  volume={15},
  pages={1-12}
}
BackgroundBacillus subtilis, a Gram-positive organism, has been developed to be an attractive expression platform to produce both secreted and cytoplasmic proteins owing to its prominent biological characteristics. We previously developed an auto-inducible expression system containing the srfA promoter (PsrfA) which was activated by the signal molecules acting in the quorum-sensing pathway for competence. The PsrfA promoter exhibited the unique property of inducer-free activity that is closely… CONTINUE READING
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