Development of an asymmetric PCR-ELISA typing method for citrus tristeza virus based on the coat protein gene.

@article{Nolasco2009DevelopmentOA,
  title={Development of an asymmetric PCR-ELISA typing method for citrus tristeza virus based on the coat protein gene.},
  author={Gustavo Nolasco and C. S. dos Santos and Gonçalo Silva and Filomena Fonseca},
  journal={Journal of virological methods},
  year={2009},
  volume={155 2},
  pages={97-108}
}
The coat protein gene of isolates of citrus tristeza virus (CTV) from 20 citrus-producing regions around the world was amplified by RT-PCR, TA cloned, and characterized by SSCP. Haplotypes that produced different patterns within each geographic region were sequenced and a database of 153 accessions of CTV was assembled. Phylogenetic analysis revealed the existence of seven well-defined clusters (Coefficient of differentiation 0.78). An asymmetric PCR-ELISA typing (APET) assay was developed in… CONTINUE READING
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