Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR.

@article{Sylvester2004DevelopmentOA,
  title={Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR.},
  author={John T. Sylvester and Sanjay K. R. Karnati and Zhongtang Yu and Mark Morrison and Jeffrey L. Firkins},
  journal={The Journal of nutrition},
  year={2004},
  volume={134 12},
  pages={3378-84}
}
Currently used microbial markers cannot distinguish protozoal nitrogen (N) from bacterial N, thus limiting research on protozoal quantification in vivo by the lack of a repeatable, accurate marker for protozoal N. We report the development of a real-time PCR assay targeting the gene encoding 18S rDNA to quantify the amount of protozoal biomass in ruminal fluid and duodenal digesta. Protozoal cells were harvested from rumen fluid and concentrated for evaluation of recovery of rDNA in samples… CONTINUE READING

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