Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR.

@article{Sylvester2004DevelopmentOA,
  title={Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR.},
  author={John T Sylvester and Sanjay K R Karnati and Zhongtang Yu and Mark Morrison and Jeffrey L. Firkins},
  journal={The Journal of nutrition},
  year={2004},
  volume={134 12},
  pages={3378-84}
}
Currently used microbial markers cannot distinguish protozoal nitrogen (N) from bacterial N, thus limiting research on protozoal quantification in vivo by the lack of a repeatable, accurate marker for protozoal N. We report the development of a real-time PCR assay targeting the gene encoding 18S rDNA to quantify the amount of protozoal biomass in ruminal… CONTINUE READING