Development of an allele-specific PCR for detection of the K65R resistance mutation in patients infected with subtype C human immunodeficiency virus type 1.

@article{Toni2010DevelopmentOA,
  title={Development of an allele-specific PCR for detection of the K65R resistance mutation in patients infected with subtype C human immunodeficiency virus type 1.},
  author={Thomas A Toni and Bluma G Brenner and Eugene Lekeawung Asahchop and Michel Ntemgwa and Daniella Moisi and Mark A. Wainberg},
  journal={Antimicrobial agents and chemotherapy},
  year={2010},
  volume={54 2},
  pages={907-11}
}
The selection of drug-resistant variants of human immunodeficiency virus type 1 (HIV-1) is an impediment to the efficiency of antiretroviral (ARV) therapy. We have developed an allele-specific real-time PCR assay to explore the presence of K65R minority species among treated HIV-1 subtype B and C infections. Thirty HIV-1 subtype C- and 26 subtype B-infected patients lacking K65R as determined by conventional sequencing methods were studied, and viral minority species were found in four HIV-1… CONTINUE READING