Development of an acetaminophen amperometric biosensor based on peroxidase entrapped in polyacrylamide microgels.

@article{GonzlezSnchez2011DevelopmentOA,
  title={Development of an acetaminophen amperometric biosensor based on peroxidase entrapped in polyacrylamide microgels.},
  author={M. Isabel Gonz{\'a}lez-S{\'a}nchez and Jorge Rubio-Retama and Enrique L{\'o}pez-Cabarcos and Edelmira Valero},
  journal={Biosensors & bioelectronics},
  year={2011},
  volume={26 5},
  pages={1883-9}
}
In this work, horseradish peroxidase (HRP) has been entrapped in cross-linked polyacrylamide microparticles using the concentrated emulsion polymerization method. The feasibility of amperometric detection of acetaminophen (APAP) in a biosensor using this HRP immobilized system as the biological material in the presence of hydrogen peroxide was investigated. We found that the optimum microgel cross-linking degree required to retain the protein and to allow the diffusion of the phenolic drug onto… CONTINUE READING

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