Development of an XTT tetrazolium salt-based assay for detection of specific hyperthermia sensitizers in a high-flux screening programme.

@article{Lechpammer2002DevelopmentOA,
  title={Development of an XTT tetrazolium salt-based assay for detection of specific hyperthermia sensitizers in a high-flux screening programme.},
  author={Stanislav Lechpammer and Alexzander Asea and Reema Mallick and Rui Zhong and Michael Yu Sherman and Stuart K. Calderwood},
  journal={International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group},
  year={2002},
  volume={18 3},
  pages={203-15}
}
It is now possible to search for new drugs using high-throughput screening of chemical libraries accumulated over the past few years. To detect potential new hyperthermia sensitizers, we are screening for chemical inhibitors of thermotolerance. For the screening of a large chemical library, a rapid and simple assay based on the XTT-tetrazolium salt with the addition of intermediate electron acceptor, phenazine methosulphate (PMS) as a promoter, was developed. It was found that the sensitivity… CONTINUE READING

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