Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.

@article{Shiraiwa2015DevelopmentOA,
  title={Development of an SNP-based PCR assay for rapid differentiation of a Japanese live vaccine strain from field isolates of Erysipelothrix rhusiopathiae.},
  author={Kazumasa Shiraiwa and Yohsuke Ogawa and Masahiro Eguchi and Hirokazu Hikono and Masahiro Kusumoto and Y. Shimoji},
  journal={Journal of microbiological methods},
  year={2015},
  volume={117},
  pages={11-3}
}
The differentiation of vaccine from non-vaccine isolates is important for disease control. Based on single nucleotide polymorphisms identified by comparison of the genomes of the Koganei 65-0.15 vaccine strain and a reference strain of Erysipelothrix rhusiopathiae, we developed a PCR assay that can differentiate the vaccine strain from field isolates.