Development of an ELISA using anti-idiotypic antibody for diagnosis of opisthorchiasis.

@article{Bulashev2016DevelopmentOA,
  title={Development of an ELISA using anti-idiotypic antibody for diagnosis of opisthorchiasis.},
  author={Aitbay K. Bulashev and Sergey Borovikov and Shynar S Serikova and Zhanbolat A Suranshiev and Vladimir S. Kiyan and Saule Z. Eskendirova},
  journal={Folia parasitologica},
  year={2016},
  volume={63}
}
Monoclonal antibody specific for an epitope of cretory-secretory antigen protein of Opisthorchis felineus (Rivolta, 1884) (Trematoda: Opisthorchiidae) with a molecular weight of 28 kDa was used in a sandwich enzyme-linked immunosorbent assay (ELISA) for immobilisation of liver fluke specific antigen to the solid phase. Examination of human sera by this ELISA compared with commercial assays demonstrated that the monoclonal antibody epitope is located within this significant parasite protein… Expand
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