Development of an ELISA using a recombinant 41 kDa partial protein (P45N') for the detection of Riemerella anatipestifer infections in ducks.

@article{Huang2002DevelopmentOA,
  title={Development of an ELISA using a recombinant 41 kDa partial protein (P45N') for the detection of Riemerella anatipestifer infections in ducks.},
  author={Bin Huang and Jimmy Kwang and Hilda Loh and Joachim Frey and Hao Tan and K Chua},
  journal={Veterinary microbiology},
  year={2002},
  volume={88 4},
  pages={
          339-49
        }
}
Riemerella anatipestifer, a gram-negative bacillus, is the causative agent of duck septicemia, a disease which could incur much economic loss in the duck industry. An indirect enzyme-linked immunosorbent assay (ELISA) has been developed to facilitate early detection of R. anatipestifer infection in ducks. The antigen used was a recombinant 41 kDa N-terminal fragment (rP45N') of a newly characterized R. anatipestifer potential surface protein, P45, which was expressed in Escherichia coli as an N… CONTINUE READING