Development of a whole-cell biosensor by cell surface display of a gold-binding polypeptide on the gold surface.

@article{Park2009DevelopmentOA,
  title={Development of a whole-cell biosensor by cell surface display of a gold-binding polypeptide on the gold surface.},
  author={Tae Jung Park and Shun Zheng and Yeon Jae Kang and Sang Yup Lee},
  journal={FEMS microbiology letters},
  year={2009},
  volume={293 1},
  pages={141-7}
}
Cell surface display was used as a strategy to display the gold-binding polypeptide (GBP) fusion protein on the surface of Escherichia coli, and consequently to immobilize the cells on the gold surface. The DNA encoding the GBP was fused to the truncated fadL gene and was expressed by the tac promoter. For the display of the core streptavidin (cSA) of Streptomyces avidinii, the cSA gene was fused to the truncated oprF gene. After the dual display of FadL-GBP and OprF-cSA on the surface of E… CONTINUE READING

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