Development of a whole blood intracellular cytokine staining assay for mapping CD4(+) and CD8(+) T-cell responses across the HIV-1 genome.

@article{MeddowsTaylor2007DevelopmentOA,
  title={Development of a whole blood intracellular cytokine staining assay for mapping CD4(+) and CD8(+) T-cell responses across the HIV-1 genome.},
  author={Stephen Meddows-Taylor and Sharon Shalekoff and Louise Kuhn and Glenda Elisabeth Gray and Caroline T Tiemessen},
  journal={Journal of virological methods},
  year={2007},
  volume={144 1-2},
  pages={
          115-21
        }
}
A whole blood peptide mapping intracellular cytokine staining (ICS) assay was developed that allows the direct comparison, at the individual peptide level, of CD4(+) and CD8(+) T-cell responses that span every encoded protein, in patients infected with HIV-1. Whole blood samples from HIV-1 infected patients were stimulated with overlapping synthetic peptides spanning nine subtype C HIV-1 gene regions (Gag, Pol, Nef, Env, Tat, Rev, Vif, Vpu, Vpr). Following stimulation and permeabilization… CONTINUE READING
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