Development of a walleye spleen stromal cell line sensitive to viral hemorrhagic septicemia virus (VHSV IVb) and to protection by synthetic dsRNA.

@article{Vo2015DevelopmentOA,
  title={Development of a walleye spleen stromal cell line sensitive to viral hemorrhagic septicemia virus (VHSV IVb) and to protection by synthetic dsRNA.},
  author={Nguyen T K Vo and Aaron W. Bender and Dustin A Ammendolia and John S Lumsden and Brian Dixon and Niels C. Bols},
  journal={Fish & shellfish immunology},
  year={2015},
  volume={45 1},
  pages={
          83-93
        }
}
A cell line, WE-spleen6, has been developed from the stromal layer of primary spleen cell cultures. On conventional plastic, WE-spleen6 cells had a spindle-shaped morphology at low cell density but grew to become epithelial-like at confluency. On the commercial extracellular matrix (ECM), Matrigel, the cells remained spindle-shaped and formed lumen-like structures. WE-spleen6 cells had intermediate filament protein, vimentin and the ECM protein, collagen I, but not smooth muscle α-actin (SMA… CONTINUE READING

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