Development of a versatile procedure based on natural transformation for marker-free targeted genetic modification in Streptococcus thermophilus.

@article{Fontaine2010DevelopmentOA,
  title={Development of a versatile procedure based on natural transformation for marker-free targeted genetic modification in Streptococcus thermophilus.},
  author={Laetitia Fontaine and Damien Dandoy and C{\'e}line Boutry and Brigitte Delplace and Marie Henry de Frahan and Christophe Fremaux and Philippe Horvath and Patrick Boyaval and Pascal Hols},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 23},
  pages={7870-7}
}
A versatile natural transformation protocol was established for and successfully applied to 18 of the 19 Streptococcus thermophilus strains tested. The efficiency of the protocol enables the use of in vitro-amplified mutagenesis fragments to perform deletion or insertion of large genetic fragments. Depending on the phenotype linked to the mutation, markerless mutants can be selected either in two steps, i.e., resistance marker insertion and excision using an adapted Cre-loxP system, or in one… CONTINUE READING

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