Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.

@article{McDonald2005DevelopmentOA,
  title={Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.},
  author={Ryan R. McDonald and Nick A Antonishyn and Toni Hansen and Laelie A. Snook and Evelyn Nagle and Michael R. Mulvey and Paul N. Levett and Greg B. Horsman},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 12},
  pages={6147-9}
}
Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA. 

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