Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants

@article{Lima2003DevelopmentOA,
  title={Development of a transformation system for Crinipellis perniciosa, the causal agent of witches' broom in cocoa plants},
  author={Juliana de Oliveira Roque E Lima and Jildete Karla dos Santos and Jorge Fernando Pereira and M{\'a}rio L{\'u}cio Vilela de Resende and Elza Fernandes de Ara{\'u}jo and Marisa Vieira de Queiroz},
  journal={Current Genetics},
  year={2003},
  volume={42},
  pages={236-240}
}
Protoplasts of the pathogenic plant fungus, Crinipellis perniciosa, were transformed to hygromycin B resistance using the pAN7-1 plasmid, which contains the Escherichia coli hph gene under the control of Aspergillus nidulans regulatory sequences. The pAN7-1 plasmid was introduced by PEG/CaCl2 treatment. Transformation frequencies of 1.6–2.5 transformants/μg… CONTINUE READING