Development of a technique to quantify the effectiveness of enrichment regimes in recovering "stressed" Listeria cells.

  title={Development of a technique to quantify the effectiveness of enrichment regimes in recovering "stressed" Listeria cells.},
  author={Carolyn M. Osborne and Phil J. Bremer},
  journal={Journal of food protection},
  volume={65 7},
A rapid, reliable microwell plate method based on the most probable number (MPN) technique was used to determine the effectiveness of five enrichment regimes in the recovery and enumeration of Listeria spp. cells from five seafood products. The products tested were chosen to reflect conditions under which cells were exposed to the "stresses" associated with a variety of food-processing techniques, such as treatments involving an ethanol-based marinade, lowered pH (acetic acid), heat, sugar and… 
Profiling of recovery efficiencies for three standard protocols (FDA-BAM, ISO-11290, and modified USDA) on temperature-injured Listeria monocytogenes.
Results indicate that the mUSDA method is best able to aid the recovery of heat-injured, freeze- injured, and heat-freeze-in injured cells and was shown to be the most cost effective for heat- Freezing-Injured cells.
Research Issues in Inactivation of Listeria monocytogenes Associated with New Zealand Greenshell Mussel Meat (Perna canaliculus) Using High-Pressure Processing
New Zealand Greenshell mussels are currently shucked by heat processing, and this can be used as a listericidal step, but processors need to understand the effects of this technology on the safety of their product with respect to Listeria monocytogenes.
Comparison of Subtypes of Listeria monocytogenes Isolates from Naturally Contaminated Watershed Samples with and without a Selective Secondary Enrichment
Evidence is provided that using both enrichment culture methods allowed for detection of a greater diversity of isolates among the samples than the use of one method alone, and that a wide diversity of L. monocytogenes strains exist in this watershed.
Evaluation of the effectiveness of a commercially available defined substrate medium and enumeration system for measuring <i>Escherichia coli</i> numbers in faeces and soil samples<link href='#fn1'>*</link>
R.W. MUIRHEAD, R .P . L ITTLEJOHN AND P.J . BREMER. 2004. Aims: To determine if a commercially available defined substrate medium and enumeration system could be utilized as an effective and accurate
Detection of low numbers of healthy and sub-lethally injured Salmonella enterica in chocolate.


Efficacy of Enrichment Media for Recovery of Heat-Injured Listeria monocytogenes.
The efficacy of University of Vermont (UVM) and Listeria enrichment broth (LEB) enrichment broths for the recovery of heat-injured Listeria monocytogenes was determined in pure culture and in the
Development of a simple recovery-enrichment system for enhanced detection of heat-injured Listeria monocytogenes in pasteurized milk.
Replacement of phosphate buffer with 3-[N-morpholino]propanesulfonic acid (MOPS) buffer and addition of magnesium sulfate (15 mM) enhanced recovery and detection of L. monocytogenes in one vessel in the presence of thermoduric background microflora commonly found in pasteurized milk.
Evaluation of enrichment broths for their ability to recover heat-injured Listeria monocytogenes.
Recovering heat-treated cells was significantly higher in LEB, followed by MUVM broth, UVM broth and FB, and the addition of catalase to enrichment broths significantly enhanced recovery of heat-injured cells.
Comparison of Three Selective Enrichment Methods for the Isolation of Listeria monocytogenes from Naturally Contaminated Foods.
It is suggested that at least two enrichment methods must be used in combination to recover L. monocytogenes from contaminated foods with a success rate near 90%.
Comparative recovery of uninjured and heat-injured Listeria monocytogenes cells from bovine milk
The standard selective enrichment protocols of the Food and Drug Administration (FDA) and U.S. Department of Agriculture (USDA) were compared with an experimental nonselective broth enrichment (NSB)
Development of a repair-enrichment broth for resuscitation of heat-injured Listeria monocytogenes and Listeria innocua
The ability of the divalent cations magnesium, iron, calcium and manganese; yeast extract; pyruvate; catalase; and the carbohydrates glucose, lactose, sucrose, esculin, fructose, galactose, maltose,
Current Research on Listeria monocytogenes in Foods: an Overview.
  • J. Farber
  • Biology, Medicine
    Journal of food protection
  • 1993
It appears that more countries may be moving towards establishing tolerance levels for the presence of the organisms in foods, especially for those low-risk foods which do not support growth of the organism and for those foods with a very short shelf life.
Effect of temperature, sodium chloride, and pH on growth of Listeria monocytogenes in cabbage juice
The effects of temperature, NaCl, and pH on the growth of the organism in cabbage were unknown and two strains (LCDC 81-861 and Scott A, both serotype 4b) were examined.