Development of a swine-specific fecal pollution marker based on host differences in methanogen mcrA genes.

@article{Ufnar2007DevelopmentOA,
  title={Development of a swine-specific fecal pollution marker based on host differences in methanogen mcrA genes.},
  author={Jennifer A. Ufnar and David F. Ufnar and Shiao Y. Wang and Rudolph D Ellender},
  journal={Applied and environmental microbiology},
  year={2007},
  volume={73 16},
  pages={5209-17}
}
The goal of this study was to evaluate methanogen diversity in animal hosts to develop a swine-specific archaeal molecular marker for fecal source tracking in surface waters. Phylogenetic analysis of swine mcrA sequences compared to mcrA sequences from the feces of five animals (cow, deer, sheep, horse, and chicken) and sewage showed four distinct swine clusters, with three swine-specific clades. From this analysis, six sequences were chosen for molecular marker development and initial testing… CONTINUE READING
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