Development of a surface display ELISA to detect anti-IgG antibodies against bovine αS1-casein in human sera.

Abstract

The aim of the present study was to develop a surface display ELISA (SD-ELISA) for IgG-serum reaction against bovine casein αS1 (CSN1S1). In a SD-ELISA, the antigen is displayed on the surface of Escherichia coli using the autodisplay technology and whole cells of E. coli are used to coat the microplates for serum testing. After establishing the setup of… (More)
DOI: 10.1016/j.jpba.2014.03.032

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