Development of a simple transient assay for Ac/Ds activity in cells of intact barley tissue.

@article{McElroy1997DevelopmentOA,
  title={Development of a simple transient assay for Ac/Ds activity in cells of intact barley tissue.},
  author={David McElroy and Jeanine D. Louwerse and Sheri M McElroy and Peggy G. Lemaux},
  journal={The Plant journal : for cell and molecular biology},
  year={1997},
  volume={11 1},
  pages={
          157-65
        }
}
The development of a barley (Hordeum vulgare L.) transformation system made it possible to consider the use of maize Activator/Dissociation (Ac/Ds) transposable elements for gene tagging in transgenic barley plants. However, barley transformation is time-consuming, and therefore a simple transient assay for Ac/Ds activity in intact barley tissues was developed to test the components of a proposed gene tagging system, prior to their stable introduction into plants. In this assay, barley… CONTINUE READING
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