Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype

@article{IiguezLuy2008DevelopmentOA,
  title={Development of a set of public SSR markers derived from genomic sequence of a rapid cycling Brassica oleracea L. genotype},
  author={Federico L. I{\~n}iguez-Luy and Amy M. Vande Voort and Thomas C. Osborn},
  journal={Theoretical and Applied Genetics},
  year={2008},
  volume={117},
  pages={977-985}
}
The traditional development of simple sequence repeat (SSR) or microsatellite markers by probe hybridization can be time-consuming and requires the use of specialized laboratory equipment. In this study, probe hybridization was circumvented by using sequence information on 3,500 genomic clones mainly from Brassica oleracea to identify di, tri, tetra and penta-nucleotide repeats. A total of 587 primer pairs flanking SSR were developed using this approach. From these, 420 SSR markers amplified… CONTINUE READING

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