Development of a sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction for detection of Vibrio cholerae/mimicus, V. parahaemolyticus/alginolyticus and Campylobacter jejuni/coli.

@article{Kurakawa2012DevelopmentOA,
  title={Development of a sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction for detection of Vibrio cholerae/mimicus, V. parahaemolyticus/alginolyticus and Campylobacter jejuni/coli.},
  author={Takashi Kurakawa and Hiroyuki Kubota and Hirokazu Tsuji and Kazunori Matsuda and Takashi Asahara and Takuya Takahashi and Thandavarayan Ramamurthy and Takashi Hamabata and Eizo Takahashi and Shin-ichi Miyoshi and Keinosuke Okamoto and Asish Kumar Mukhopadhyay and Yoshifumi Takeda and Koji Nomoto},
  journal={Microbiology and immunology},
  year={2012},
  volume={56 1},
  pages={
          10-20
        }
}
A sensitive rRNA-targeted reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method was developed for detection of Vibrio cholerae/mimicus, V. parahaemolyticus/alginolyticus and Campylobacter jejuni/coli by using specific primers. Counts of the enteric pathogens spiked in human stools were quantified at the lower detection limit of 10(3) cells/g stool by RT-qPCR, in marked contrast with conventional quantitative polymerase chain reaction (qPCR) at the detection limit of 10(5… CONTINUE READING
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