Development of a sensitive gene expression reporter system and an inducible promoter-repressor system for Clostridium acetobutylicum.

Abstract

A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of beta-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter… (More)

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