Development of a sensitive detection method to survey pelagic biodiversity using eDNA and quantitative PCR: a case study of devil ray at seamounts

@article{Gargan2017DevelopmentOA,
  title={Development of a sensitive detection method to survey pelagic biodiversity using eDNA and quantitative PCR: a case study of devil ray at seamounts},
  author={Laura M. Gargan and Telmo Morato and Christopher Kim Pham and John A. Finarelli and Jeanette E. L. Carlsson and Jens Carlsson},
  journal={Marine Biology},
  year={2017},
  volume={164},
  pages={1-9}
}
Environmental DNA (eDNA) is increasingly being used in aquatic environments for monitoring species, particularly those that are of conservation concern and/or are difficult to visually observe. Quantitative PCR (qPCR) has been employed to detect low abundance species occurring in environmental water samples. However, the qPCR technique has principally been applied to freshwater habitats, with less application to pelagic marine environments. We developed a species-specific eDNA assay for the… 

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