Development of a sensitive assay for detection of replication-competent recombinant lentivirus in large-scale HIV-based vector preparations.

@article{Escarpe2003DevelopmentOA,
  title={Development of a sensitive assay for detection of replication-competent recombinant lentivirus in large-scale HIV-based vector preparations.},
  author={Paul Escarpe and Nathalie Zayek and Peggy Chin and Flavia Borellini and Romain Zufferey and G{\'a}bor G{\'a}bor Veres and Veronique Kiermer},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2003},
  volume={8 2},
  pages={332-41}
}
Lentiviral vectors have demonstrated great potential as gene therapy vectors mediating efficient ex vivo and in vivo gene delivery and long-term transgene expression in both dividing and nondividing cells. However, for clinical studies it must be demonstrated that lentiviral vector preparations are safe and not contaminated by replication-competent recombinants related to the parental pathogenic virus. Here we describe a sensitive assay for the detection of replication-competent lentiviruses… CONTINUE READING

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