Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae.

Abstract

Based on use of a loop-mediated isothermal amplification (LAMP) identification protocol, this study attempted to detect Lactococcus garvieae in fish by using primer sets designed from an L. garvieae alpha/beta fold family hydrolase gene. Reaction time and temperatures were optimized for 60 min at 60°C with the resulting amplicons visualized by adding SYBR… (More)
DOI: 10.3354/dao02546

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