Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR.


The aim of the present work was to develop two new independent SYBR Green I-based real-time PCR assays for both detection and quantification of betanodavirus, an RNA virus that infects several species of marine teleost fish causing massive mortalities in larvae and juveniles. The assays utilized two pairs of primers targeting highly conserved regions of… (More)


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