Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples

@inproceedings{Zhang2012DevelopmentOA,
  title={Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples},
  author={Eileen Z. Zhang and Doug J. Bartels and James D. Frantz and Sheila D Seepersaud and Judith A Lippke and Benjamin Shames and Yi Zhou and Chao Shuang Lin and Ann D. Kwong and Tara L. Kieffer},
  booktitle={Virology Journal},
  year={2012}
}
Direct-acting antiviral (DAAs) agents for hepatitis C virus (HCV) span a variety of targets, including proteins encoded by the NS3/4A, NS4B, NS5A, and NS5B genes. Treatment with DAAs has been shown to select variants with sequence changes in the HCV genome encoding amino acids that may confer resistance to the treatment. In order to assess these effects in patients, a Reverse Transcription Polymerase Chain Reaction (RT-PCR) method was developed to sequence these regions of HCV from patient… CONTINUE READING
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