Development of a sample preparation method for fungal proteomics.

  title={Development of a sample preparation method for fungal proteomics.},
  author={Motoyuki Shimizu and Hiroyuki Wariishi},
  journal={FEMS microbiology letters},
  volume={247 1},
Since filamentous fungi including basidiomycetous fungi possess an exceptionally robust cell wall as in microorganisms, effective extraction of intracellular proteins is a key step for fungal proteomic studies. To overcome the experimental obstacle caused by cell walls, we utilized fungal protoplasts, prepared from the brown-rot basidiomycete, Tyromyces palustris. The amount and quality of proteins extracted from the protoplast cells were much higher than that from the mycelial cells… CONTINUE READING

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