Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis

@article{MohammadiYeganeh2012DevelopmentOA,
  title={Development of a robust, low cost stem-loop real-time quantification PCR technique for miRNA expression analysis},
  author={Samira Mohammadi-Yeganeh and Mahdi Paryan and Siamak Mirab Samiee and Masoud Soleimani and Ehsan Arefian and Keyhan Azadmanesh and Ehsan Mostafavi and Reza Mahdian and Morteza Karimipoor},
  journal={Molecular Biology Reports},
  year={2012},
  volume={40},
  pages={3665-3674}
}
Development of a rapid and accurate quantification method for the detection of microRNAs (miRNAs) has been desired, in particular, when they are differently expressed in normal and pathological conditions. However, various methods for the quantification of small non-coding RNAs as well as miRNAs have been described. These methods mainly include hybridization-based approaches such as primer extension, northern blotting, microarray profiling, and reverse transcription (RT) PCR. Here, we developed… CONTINUE READING

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