Development of a real-time RT-PCR assay for a novel influenza A (H1N1) virus.

@article{Jiang2010DevelopmentOA,
  title={Development of a real-time RT-PCR assay for a novel influenza A (H1N1) virus.},
  author={Tao Jiang and Xiao-ping Kang and Yongqiang Deng and Hui Zhao and Xiao-Feng Li and Xuedong Yu and Man Yu and E-de Qin and Qing-yu Zhu and Yinhui Yang and Chengfeng Qin},
  journal={Journal of virological methods},
  year={2010},
  volume={163 2},
  pages={470-3}
}
A pandemic caused by a novel influenza A virus (H1N1) poses a serious public health threat. In this study, a real-time reverse transcriptase PCR (RT-PCR) assay based on the hemagglutinin gene was developed that discriminates the novel H1N1 from swine influenza virus, seasonal H1N1/H3N2 virus and the highly pathogenic H5N1 avian influenza virus. The sensitivity of this assay was 0.2 50% tissue culture infective dose of virus and 200 copies of in vitro-transcribed target RNA. Three hundred and… CONTINUE READING

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