Development of a real-time PCR assay for the detection of Cladosporium fulvum in tomato leaves.

Abstract

AIMS The aim of this study was to develop a sensitive real-time polymerase chain reaction (PCR) assay for the rapid detection of Cladosporium fulvum in tomato leaves. METHODS AND RESULTS Three PCR primer pairs were designed based on the nucleotide sequences of: (i) the internal transcribed spacer regions of ribosomal RNA; (ii) a microsatellite region… (More)
DOI: 10.1111/j.1365-2672.2007.03660.x

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