Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification.

Abstract

A molecular diagnostic assay utilizing reverse transcription-recombinase polymerase amplification (RT-RPA) at an isothermal constant temperature of 39°C and target-specific primers and probe were developed for the rapid, sensitive, and specific detection of tomato chlorotic dwarf viroid (TCDVd) in infected leaf and seed tissues. The performance of the… (More)
DOI: 10.1016/j.jviromet.2016.06.013

Topics

Cite this paper

@article{Hammond2016DevelopmentOA, title={Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification.}, author={Rosemarie W. Hammond and Shulu Zhang}, journal={Journal of virological methods}, year={2016}, volume={236}, pages={62-67} }