Development of a rapid and sensitive method for determination of cysteine/cystine ratio in chemically defined media.

Abstract

Two rapid, sensitive and quantitative methods for the determination of the cysteine and cystine ratio in complex defined media feedstock using monolithic reversed-phase liquid chromatography (RPLC) and RPLC-MS are presented. Cysteine is pre-derivatised with purified 2-chloro-1-methylquinolinium tetrafluoroborate (CMQT) and separated from other… (More)
DOI: 10.1016/j.chroma.2010.04.036

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