Development of a quantitative bio/chemiluminescence spectrometer determining quantum yields: re-examination of the aqueous luminol chemiluminescence standard.

Abstract

We have developed a bio/chemiluminescence spectrometer with a cooled charge-coupled-device (CCD) detector to obtain a quantitative luminescence spectrum as the absolute number of all emitted photons at each wavelength. The integrated area of the spectrum divided by the number of reacted substrate molecules gives the quantum yield. Calibration of the… (More)

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@article{Ando2007DevelopmentOA, title={Development of a quantitative bio/chemiluminescence spectrometer determining quantum yields: re-examination of the aqueous luminol chemiluminescence standard.}, author={Yoriko Ando and Kazuki Niwa and Nobuyuki Yamada and Tsutomu Irie and Toshiteru Enomoto and Hidehiro Kubota and Yoshihiro Ohmiya and Hidefumi Akiyama}, journal={Photochemistry and photobiology}, year={2007}, volume={83 5}, pages={1205-10} }