Development of a qualitative real-time PCR method to detect 19 targets for identification of genetically modified organisms

@inproceedings{Peng2016DevelopmentOA,
  title={Development of a qualitative real-time PCR method to detect 19 targets for identification of genetically modified organisms},
  author={Cheng Peng and Pengfei Wang and XiaoLi Xu and Xiaofu Wang and Wei Wei and Xiaoyun Chen and Junfeng Xu},
  booktitle={SpringerPlus},
  year={2016}
}
As the amount of commercially available genetically modified organisms (GMOs) grows recent years, the diversity of target sequences for molecular detection techniques are eagerly needed. Considered as the gold standard for GMO analysis, the real-time PCR technology was optimized to produce a high-throughput GMO screening method. With this method we can detect 19 transgenic targets. The specificity of the assays was demonstrated to be 100 % by the specific amplification of DNA derived from… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 19 references

Detection of genetically modified components-Maize test methods. SN/T 1196-2012 Standards

  • JJ Cao, JY Xu
  • 2012

Detection of genetically modified organisms and derived products-qualitative PCR methods based on nucleic acid

  • LW Pan, WJ Zhen
  • 2012

Global status of commercialized biotech/GM Crops

  • C James
  • ISAAA brief no
  • 2012
1 Excerpt

Protocol of polymerase chain reaction for detecting genetically modified components in cotton

  • H Liu, W Liu, Y He
  • 2010

Application of stepwise ammonium sulfate precipitation as cleanup tool for an enzymelinked immunosorbent assay of glyphosate oxidoreductase in genetically modified rape of gt73

  • W Xu, K Huang, +4 authors Y Luo
  • J Food Biochem
  • 2009

Similar Papers

Loading similar papers…